The substantiation of the optimal ph range of the preventive spray for veterinary
Keywords:antiseptic spray, veterinary drug, silver citrate, copper citrate, pH, stability
Aim. To determine the optimal pH range of the original combined veterinary drug in the dosage form of the spray in order to provide the stability of the solution during the shelf life.
Materials and methods. The study object was the samples of the spray with the content of silver and copper citrate, dexpanthenol, and excipients. The quantitative content of silver ions was determined by the thiocyanometric method. The quantitative content of copper ions was determined according to the method of the State Pharmacopoeia of Ukraine 2.0 (SPhU) “2.2.25 Absorption spectrophotometry in the ultraviolet and visible areas”. The quantitative determination of dexpanthenol was carried out by liquid chromatography. The potentiometric determination of pH was carried out using the method of the SPhU.
Results. As the result of the studies conducted it has been found that the sample with the pH of 5.32 produces a blue precipitate and an opalescence of a grayish tint. Over time (24 h) opalescence does not disappear, and the precipitate acquires a gray tint. This fact indicates destruction of copper citrate due to the increase in pH without addition of the excessive citric acid; it leads to formation of copper oxide hydrate. It can also be assumed that the stability of silver citrate in the solution is impaired, and metallic silver particles appear in the form of a precipitate. In these samples the quantitative content of silver and copper ions in the solution did not correspond to the limits set. Samples with the pH from 1.80 to 5.05 corresponded to the limits set for the content of silver ions, copper ions and dexpanthenol, and there were no changes in the appearance of the solution after pH correction.
Conclusions. According to the results obtained the optimal pH range (2.0-5.0) for the solution with the antimicrobial activity under the conventional name “Argocide-copper” – a drug based on silver citrate, copper citrate and dexpanthenol has been determined. This pH level makes it possible to obtain a stable drug. The studies conducted will be used when developing the spray with the antiseptic action for application in veterinary medicine and introducing it into production.
Mazurkevich, A. Y., Grishchuk, A. V. (2017). Visnyk Dnipropetrovskoho derzhavnoho ahrarno-ekonomichnoho universytetu, 3 (45), 82–84.
Múnera-Bedoya, O. D., Cassoli,L. D., Machado, P. F., Cerón-Muñoz, M. F.(2017). Influence of attitudes and behavior of milkers on the hygienic and sanitary quality of milk. Journal of Dairy Science, 12 (9), 86–92. doi: 10.1371/journal.pone.0184640.
Baryshev, V. A., Popova, O. S., Tokareva, O. A. (2017). Uchenyie zapiski uchrezhdeniia obrazovaniia “Vitebskaia ordena “Znak Pocheta” gosudarstvennaia akademiia veterinarnoy meditsyny”, 53( 2), 6–9.
Shabunin, S. V., Klimov, N. T., Nezhdanov, A. G., Efanova, L. I. (2011). Veterinariia, 12, 3–6.
Sobko, H. V. (2016). Biolohiia tvaryn, 18 (4), 86–92.
Baumberger, C., Guarín, J. F., Ruegg, P. L. (2016). Effect of 2 different premilking teat sanitation routines on reduction of bacterial counts on teat skin of cows on commercial dairy farms. Journal of Dairy Science, 99 (4), 2915–2929. doi: 10.3168/jds.2015-10003.
Danilevskaia, N. V., Arisov, M. V. Belyh, I.P., Indiuhova, E. N. (2017). Veterinarnyi vrach, 4, 40–44.
Galton, D. M. (2004). Effects of an Automatic Postmilking Teat Dipping System on New Intramammary Infections and Iodine in Milk. Journal of Dairy Science, 87, 225–231.
Kuzmich, R. G., Mironchuk, S. V., Hodykin, D. S. (2016). Retseptura v veterinarnom akusherstve I ginekologii. Vitebsk: VGAVM, 109.
Obrabotka vymeni korov. Avialable at: http://www.agrocounsel.ru/obrabotka-vymeni-korov.
Polova, Zh. M. (2016). Zbirnyk naukovykh prats spivrobitnykiv NMAPO imeni P. L. Shupyka, 25 (1), 563–568.
Polova, Zh. M., Nazarkina, V. M. (2017). Sotsialna farmatsiia v okhoroni zdorovia, 3 (3), 35–41. doi: 10.24959/sphhcj.17.86.
Derzhavne pidpryiemstvo “Ukrainskyi naukovyi ekspertnyi farmakopeinyi tsentr yakosti likarskykh zasobiv” (2015). Derzhavna farmakopeia Ukrainy. (Vols 1–3, vol. 1). (2-e ed.). Kharkiv, 1128.
Copyright (c) 2018 National University of Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).