The study of release kinetics of active pharmaceutical ingredients from a film-forming aerosol using the in vitro method
DOI:
https://doi.org/10.24959/sphhcj.20.182Keywords:
drug, kinetic studies, wound healing, aerosol, ofloxacin, miramistin, anestesinAbstract
Aim. To conduct the pharmacokinetic studies by dialysis through a semi-permeable membrane relative to the release of active pharmaceutical ingredients from the aerosol having the antibacterial, anti-inflammatory and analgesic effects with ofloxacin, miramistin and anestesin under the conditional name “AMO-sol” for the treatment of the purulent-inflammatory phase of the wound process.
Materials and methods. When conducting the experimental studies the authors used such active pharmaceutical ingredients as ofloxacin, anestesin, miramistin, as well as excipients – sodium carboxymethyl cellulose, methyl cellulose, polyvinyl alcohol, polyvinyl pyrolidone, propylene glycol, glycerin, polyethylene oxide-400, ethyl alcohol, citric acid monohydrate, сhladon-134a. The kinetic parameters of the drug were determined by dialysis through a semi-permeable membrane. For this purpose, a chamber, which consisted of two cylinders with a diameter of 50 and70 mm each, respectively, was used. The kinetic properties of the samples were studied using a biological membrane (degreased intestine) at a temperature of (34 ± 1) °C.
Results. The kinetic studies were performed by dialysis through a semi-permeable membrane to release active substances from the medicinal product (aerosol) studied in order to determine pharmacokinetic parameters (the release reaction rate, the rate constant and half-life). The half-life calculations have shown that this indicator for anesthesin grows from 127.85 s to 8783.27 s, for miramistin – from 182.37 s to 1985.67 s, for ofloxacin – from 315 s to 2924.05 s. The results obtained indicate that with an increase in the half-life the rate of the elimination process decreases. As a result of the experimental studies of the kinetic parameters of the drug and the analysis of the data obtained it has been found that the release of active pharmaceutical ingredients from the aerosol is slow at first, while over time their release from the drug increases.
Conclusions. Taking into account the impact of pharmaceutical and biological variable factors on the degree of effectiveness of drugs the results of our studies of pharmacokinetic parameters can be used as the basis for the development of an aerosol having the antibacterial, anti-inflammatory and analgesic effects with ofloxacin, miramistin and anestesin under the conditional name “AMO-sol” for the treatment of purulent inflammatory phase of the wound process.References
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