The study of the composition of the lipophilic hop extract for using in the composition of finished dosage forms




hop infructescences, lipophilic extract, gas chromatography, finished dosage forms


In the industrial production of the hop infructescence dry extract, the non-polar fraction in the form of a lipophilic extract obtained by extraction with heptane or hexane remains as a waste. The lipophilic extract obtained under such conditions is characterized by a significant content of biologically active substances from the groups of terpene alcohols, aldehydes, ketones, epoxides, acids, and esters. In this case, it is promising to use the lipophilic extract in further research on the development of new drugs, and it will also provide a comprehensive treatment of hop infructescences.

Aim. To study the chemical composition of the lipophilic hop extract by gas chromatography for further standardization and use as a constituent of finished dosage forms.

Materials and methods. The lipophilic hop extract was obtained at the SPC “Borshchagovsky CPP”, Kyiv. The chemical composition of the lipophilic fraction of hop infructescences was studied according to the requirements of the State Pharmacopoeia by gas chromatography with a flame ionization detector using an Agilent 7890 GC gas chromatograph with a 597 Agilent GC/MSD mass spectrometric detector.

Results. According to the results of the studies conducted, using library spectra, 47 volatile compounds were identified in the lipophilic hop extract. The main components were (S)-3-ethyl-4-methylpentanol, 1,3,3-trimethyl-bicyclo [2,2,1] heptane-2-one, eicosamethyl-cyclodecasiloxane, 4-pentene-2-ol, Z-7-hexadecanoic acid, phthalic acid esters, 7-tetradecenal, CIS-11-hexadecenal, their content reached 75% of the area of all peaks of substances.

Conclusions. In the lipophilic hop extract, the content of volatile substances has been identified and quantified by gas chromatography with a mass spectrometric detector. The major representatives of the substances are (S)-3-ethyl-4-methylpentanol and 1,3,3-trimethyl-bicyclo[2,2,1]heptane-2-one, their content is 49.5 % of the total amount of substances.

Author Biographies

Ie. V. Gladukh, National University of Pharmacy of the Ministry of Health of Ukraine

Doctor of Pharmacy (Dr. habil.), professor of the Department of Pharmaceutical Technology of Drugs

M. G. Podorozhna, National University of Pharmacy of the Ministry of Health of Ukraine

postgraduate student of the Department of Pharmaceutical Technology of Drugs

O. V. Bevz, National University of Pharmacy of the Ministry of Health of Ukraine

Candidate of Pharmacy (Ph. D.), teaching assistant of the Department of Medicinal Chemistry


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